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The choice of titration for analysis depends on the sensitivity required.With the advent of more improved separation methods and better alternate assay techniques bioassay have become less significant in quality control of pharmaceuticals.Formulated drugs contain certain amounts of active ingredients that have to be efficacious when used.Nonetheless, the potency of the drug depends primarily on the rate of release of the active ingredients from the formulation into active sites and also its biological availability.It has an added advantage over the classical methods by being more accurate and precise, less time in analysis, and measures in the concentration range of nano and below.For instance chromatographic separations often at times require other specific detectors to elucidate detection.These include HPLCMS, HPLCEC, GCMS, HPLCFTIR and GCFTIR.Instances where a sufficiently larger number of similar units are to be subjected routinely to the same type of examination, automated methods of analysis may be far more efficient and precise than manual methods.Automated methods have been found useful in testing the content uniformity of tablets and capsules.It has now become more convenient for establishments and well organized laboratories to utilize automated methods as alternatives to pharmacopoeial methods.Additionally, the detection system and calculation of results are often computerised.Even though automated methods are extensively used as an alternative to pharmacopoeial methods, the need for precision and accuracy has to be ascertained before their usage.In the absence of other physicochemical factors, the absorbance through which the radiation passes and to the concentration of the substance in solution in accordance with the equation: A ecb e molar absorptivity, ifbis expressed in centimetres andcin moles per litre.In double beam instrument, the light split into two beams, one beam passes through the sample and the other is used as a reference.The transparent cell used to hold the sample, called the cuvette, may be a quartze, glass or plastic.Glass and most plastics absorb in the UV, so can only be used for visible wavelengths.Chromatography involves a sample being dissolved in a mobile phase. The mobile phase is then forced through an immobile, immiscible   stationary phase.The phases are chosen such that components of the sample have differing solubilities in each phase.A component which is quite soluble in the stationary phase will take longer to travel through it than a component which is not very soluble in the stationary phase but very soluble in the mobile phase.The distribution of analytes between phases can often be described quite simply.An analyte is in equilibrium between the two phases.The equilibrium <a href="http://www.targetmol.com/compound/L-Valine"></a> constant, K, is termed the partition coefficient; defined as the molar concentration of analyte in the stationary phase divided by the molar concentration of the analyte in the mobile phase.The time between sample injection and an analyte peak reaching a detector at the end of the column is termed the retention time. Each analyte in a sample will have a different retention time.The time taken for the mobile phase to pass through the column is called the void volume, a compound which does not partition appreciably into the mobile phase also elutes at this time A term called the retention or capacity factor, k, is often used to describe the migration rate of an analyte on a column.

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