Interactions between arbuscular mycorrhizal fungi and bacteria and their potential for stimulating plant growth.times in control treatment.Optimal exploitation from these sources not only affects on soil properties positively, but also useful in terms of economic, social, and environmental aspects and it may serve as a suitable substitute for chemical products. The phosphate solubilizing bacteria are known as the factors for rising rate of phosphorus absorption that their use in form of biofertilizers may improve soil nutritional status, secretion of plant growth regulators, and control of soilborne diseases and eventually it may lead to better growth and yield in farming plants. The results from studies done by many researchers indicate that these materials stimulate growth in plant by various mechanisms including production of plant hormones, increase in phosphorus absorption in plant, fixation of nitrogen, production of antibiotics, siderophores as well as secretion of enzymes, which regulate amount of ethylene in the plant. The microorganisms colonizing plant roots generally include bacteria, algae, fungi, protozoa and actinomycetes.Of different microbial populations present in the rhizosphere, bacteria are the most abundant microorganisms. PGPR play a significant role in enhancing plant growth and development both under nonstress and stress conditions by a number of direct and indirect mechanisms. Similarly, some of these bacteria may release significantly useful minerals like phosphorus, potassium, magnesium, manganese, iron, copper, and zinc and thus to change culture medium and plant growth. The efficiency of growth stimulating bacteria depends on potential of colonization in rhizome system and removal of hazardous microorganisms and or the plant pathogens as well as germination potential and regeneration in host plant.An active root system releases regularly organic compounds into plant rhizome environment. The role of such microorganisms in rising absorption of nutrients has been emphasized in many researches.These researchers found that multiple inoculation of tomato with mycorrhiza fungi and phosphate solubilizing bacteria increases phosphorus and nitrogen absorption in leaves.It has reported that the growth stimulating bacteria also provide improvement condition for some of growth parameters in shoot and root organs in addition to preparation of conditions for absorption of more nutrients.So, despite of increase in cropping parameter, root biomass was decreased in some cases while some varieties increased root dry weight in depth cm.They were transferred into a sterile MS culture media and were exposed to hour photoperiod and C temperature regimes for weeks.After days, the single stem plantlets were subcultured and micropropagated on the same medium to produce sufficient numbers of plantlets for the experimental trials.These plantlets were transferred to the greenhouse and its minitubers were harvested days after planting.In this experiment, inoculation was done with bacteria in greenhouse and during transferring the resultant minitubers from single nodules into pots.Factorial experimental with completely randomized design was used in the experimental project.To implement this project, pots were considered totally each of them had two plants.These minitubers were <a href="http://www.targetmol.com/compound/Chlorpheniramine-maleate"></a>
inserted in pots with cm diameter and depth cm that was filled with mixture of perlite and sterile peat moss with ratios: to halffull.Only one minituber was kept ten days after insertion of minitubers in any vase.