Except for BF and BF formulations, all treatments tested significantly increased shootleaf weight of tea sapling.In both years, trunk diameter, plant height, shootleaf weight, fresh and dry leaf weight were greatest with the inoculation of BF, whereas the highest levels of second and third leaf area, chlorophyll. Previous studies demonstrated the application of PGPR in soil have resulted in significant increase in <a href="https://www.ncbi.nlm.nih.gov/pubmed/25062010"></a>
growth of young tea bushes and help in the reduction of the use of chemicals in tea plantations. Except for BF and BF formulations, all treatments increased glutathione reductase. In the case of increasing glucosephosphate dehydrogenase were the most effective, followed by BF.In general, GR, GST, PGD and DHSH activities were greatest with the application of BF, whereas the highest levels of POD and ADH activities were determined in treatments with BF. BF inoculations and fertilizer application caused maximum enhancement in the growth and leaf yield of tea, while NPK application and BF inoculation were the most effective promoter of PPO activity.The inoculation with some of the multitraits rhizobacterial formulationsenhanced seedling growth and defense related enzymes, such as, GR, GST, POD, and PPO in tea leaves.Since inoculation caused a differential increase in leaf defence and qualityrelated enzymes like ADH and PPO activity, as well as activation of other plant enzymes, this may indicate that activation of these enzymes in tea leaves would be differentially affected by different formulations.To alleviate stress, plants have evolved an effective antioxidant system composed of antioxidant enzymes such as GR, GST, and POD.Glucosephosphate dehydrogenase catalyse the biosynthesis of polyphenols is important in the biosynthesis of flavonoid compounds. The synthesis of the flavonoids in tea requires enzymes of the shikimate pathway, and the DHSK reductase is a key regulatory enzyme in the process.Polyphenol oxidase and peroxidase are thought to play a role in the fermentation process, oxidation and formation of the black tea compounds. Also, they are lead to the formation of black tea polyphenols and aroma compounds characteristic of black tea and involved in defense mechanism of plants against environmental stresses. Polyphenols, which are antioxidant compounds, are the major category of secondary metabolites in tea plants. Phenolic compounds are responsible for certain black tea characteristics, such as color and taste.In conclusion, inoculation with rhizobacteriabased bioformulations might have induced a greater amount of enzymes and thereby simultaneously increased growth and yield would have been recorded.Bacterialformulations caused high growth, leaf weight and enzymes activity, but it was strongly dependent on the inoculant strain formulations and parameters evaluated.Our results could be important on the tea growth, yield and quality, processing technology, and stress tolerance, in terms of PGPR research will be conducted in the future.Additional studies are required to explain the mechanism by which PGPR affects the tea quality and antioxidant enzyme responses.Growth promotion of plants by plant growthpromoting rhizobacteria under greenhouse and two different field soil conditions.The influence of plant growthpromoting rhizobacteria on growth and enzyme activities in wheat and spinach plants.Enzyme activities and growth promotion of spinach by indoleacetic acidproducing rhizobacteria.The effect of single and mixed cultures of plant growth promoting bacteria and mineral fertilizers on tea growth, yield and nutrient uptake.