Silica gel was used as desiccant, maintaining the relative humidity inside the desiccator below. Samples were heated from C at C min, under a mL min flow of N gas.An inlet port into the posterior cavity near the membrane allows a ocular mimicking flow to be established through the model.A sample cut from the fiber mat was placed in the posterior cavity and the model was reassembled.For these studies, both anterior and posterior cavities of the model were filled with phosphate buffered saline through the injection port.The inlet port was connected to a channel peristaltic pump and PBS flowed through the model at a flow rate of. m filter before analysis using highperformance liquid chromatography. A calibration curve was first constructed for each drug, and the concentrations of unknown samples were back calculated from these data.Samples from each time point were evaluated in triplicate and the mean and standard deviation were determined.Three independent experiments were conducted for each formulation, and the results are reported as mean S.For the singledrug fibers, there is evidence for incomplete evaporation of solvent during the electrospinning process, in the form of merged fibers.However, there appears to be no significant amounts of beading present, and the PVPABC fibers with all three drugs having been loaded all have smooth cylindrical morphologies with no evidence of any abnormalities.No clear trends emerge from the diameter data: there is no obvious relationship between the drug content in the fibers and their diameters PVP ABC PVPB PVPCIP PVPACY.It is presumed that both the amount of API present in solution and the effect each has on solution parameters such as viscosity and conductivity affect the diameter of the fibers obtained, thus leading to these complex patterns.Higher drug loadings appear to result in somewhat more homogenous distributions of fiber diameters.The PCLACY fibers show rough andor wrinkled surfaces in places, and high degrees of curvature.The PCLCIP sample has similar morphology, and additionally some particles can be seen on the fiber surfaces. The PCLB fibers are flattened owing to incomplete solvent evaporation during electrospinning.The PCLAB fibers are smooth but again some particles can be seen on the fiber surface, although these are much smaller and less frequent than those seen with PCLCIP.PCLABC comprises fibers with wrinkled surfaces, presumably as a result of incomplete solvent evaporation during electrospinning.In general, the multidrug fibers have higher quality than the singledrug systems, being virtually free of particles. As before, there is no direct correlation between the fibers API content and their diameters. The raw polymers PVP and PCL are amorphous and semicrystalline respectively.PVP shows a broad endotherm below C in its thermogram, corresponding to dehydration.CIP is also a crystalline material; melting is clearly visible at ca.A close inspection of all the DSC <a href="http://www.targetmol.com/compound/Abiraterone-Acetate">sell
Abiraterone Acetate</a> traces for the PCL fibers suggests traces of crystallinity are present; a particularly marked endothermic peak is present for the ACY fibers at C, indicating the possible presence of form VI of ACY. The CIP and ACY starting materials are evidently crystalline, while B is amorphous.The XRD patterns of the PCL fibers show the distinctive reflections of PCL, with some additional peaks also visible.